Hyaluronidase Inhibitor Screening Assay Kit (DIHY-100)

HYALURONIDASES are a family of enzymes that catalyze the degradation of the glycosaminoglycan, hyaluronic acid. Hyaluronic acid is one of the major constituents of the extracellular matrix in organisms where it contributes to both cell proliferation and migration. The role of hyaluronidases in breaking down this key factor in cell proliferation makes them a popular research target. BioAssay Systems' Hyaluronidase Inhibitor Screening Assay Kit uses a two step turbidimetric reaction to measure hyaluronidase activity by the amount of hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction and forms turbidity with any residual hyaluronic acid in the well. The decrease in turbidity at 600 nm is directly proportional to hyaluronidase activity in the sample.

• Rapid and reliable. Entire assay can be completed in 30 min.
• Simple and Convenient. Simple procedure with an enzymatic reaction and addition of stop reagent. No wash or reagent transfer steps involved.
• Robust and amenable to HTS. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.